معماری

Molecular detection of ESBLs production and antibiotic resistance patterns in Gram negative bacilli isolated from urinary tract infections

)Background: β-lactam resistance is more prevalent in Gram negative bacterial
isolates worldwide, particularly in developing countries. In order to provide
data relating to antibiotic therapy and resistance control, routine monitoring of
corresponding antibiotic resistance genes is necessary. Aims: The aim of this
study was the characterization of β-lactam resistance genes and its plasmid
profi le in bacteria isolated from urinary tract infection samples. Materials
and Methods: In this study, 298 Gram negative bacteria isolated from 6739
urine specimens were identifi ed by biochemical standard tests. Antimicrobial
susceptibility testing was performed by the disk diffusion method. Extendedspectrum
β-lactamase (ESBL)-producing strains were also detected by the
double-disk synergy test. The presence of blaTEM and blaSHV genes in the strains
studied was ascertained by polymerase chain reaction. Results: Of all Gram
negative bacteria, Escherichia coli (69.1%) was the most common strain,
followed by Klebsiella sp. (12.1%), Enterobacter sp. (8.4%), Proteus sp. (4.4%),
Citrobacter (4%) and Pseudomonas sp. (2%). The most antibiotic resistance was
shown to tetracycline (95.16%), nalidixic acid (89.78%) and gentamycin (73.20%)
antibiotics. Among all the strains tested, 35 isolates (11.75%) expressed ESBL
activity. The prevalence of TEM and SHV positivity among these isolates was
34.29%, followed by TEM (31.43%), TEM and SHV negativity (20.0%) and SHV
(14.29%), respectively. Conclusions: Regular monitoring of antimicrobial drug
resistance seems necessary to improve our guidelines in the use of the empirical
antibiotic therapy.

Final Released Paper(1)(1

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